The document describes Agilent's Macroporous Reversed-Phase C18 High-Recovery Protein Fractionation HPLC Column, which can achieve high protein recovery and fast desalting.
The Agilent Macroporous Reversed-Phase C18 (mRP-C18) High-Recovery Protein Fractionation Column is designed to offer greater than 95 percent protein recovery, addressing limitations of previous HPLC columns. It features a proprietary surface treatment on macroporous bonded-silica material that prevents irreversible protein adsorption.
The column offers greater than 95 percent protein recovery, with observed recoveries of greater than 98 percent for immunodepleted serum.
The column allows you to desalt in about 13 minutes, eliminating the need for a normally three-hour spin concentration process.
The mRP-C18 column has three times more loadability than conventional HPLC C8 columns, with a stated capacity of 380 ug.