Key facts
- Brand
- Agilent
- Model
- Varian 1200L LC
- Documents
- 2
- Languages
- EN
- Last added
- 2012-07-03
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Agilent Application Note #24 High-Throughput Detection of Acrylamide in Food with a Rapid Sensitive LC/MS/MS Method
The document presents an analytical method to detect acrylamide, a potential carcinogen found in cooked foods, utilizing liquid chromatography-tandem mass spectrometry (LC/MS/MS). The procedure is designed for high throughput, capable of analyzing up to 96 samples including standards within approximately five hours.
The method employs a Varian 1200L LC/MS/MS system equipped with an ESI source and a CTC Analytics HTS PAL Autosampler. Sample preparation involves extraction using internal standards followed by filtration through a Captiva kit. The analysis achieves linear calibration curves in the range of 10,000 to 20 ppb, with limits of detection at 8 ppb and quantification at 20 ppb.
Agilent Varian 1200L LC specifications
| Limits of Detection (LOD) | 8 ppb |
|---|---|
| Limits of Quantification (LOQ) | 20 ppb |
| Calibration Range | 10,000 to 20 ppb |
| Correlation Coefficient (r) | 0.994 |
| Injection Volume | 10 pL |
| Flow rate | 0.2 mL per min |
All Agilent Varian 1200L LC documents by type
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the limit of detection for acrylamide in this method?
The limit of detection is 8 ppb.
How many samples can be analyzed per run?
The method can analyze any combination of 96 samples in a little more than 5 hours.
What compounds were used as test compounds in this study?
The study used TX1360, a proprietary CNS drug, and its major hydroxylated metabolite (TX1370) as the test compounds.
What was the internal standard used for this analysis?
Buspirone was selected as the internal standard for this work.