Key facts
- Brand
- Agilent
- Model
- 6210 TOF MS
- Documents
- 3
- Languages
- EN
- Last added
- 2012-07-02
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Agilent Antibody Analysis by ESI-TOF LC/MS Application Note
Monoclonal antibodies (mAbs) are complex structures requiring accurate characterization, particularly for determining molecular weight. Traditional methods like gel analysis or MALDI-TOF often suffer from imprecision or lengthy processing times.
This study evaluates the combination of Agilent Technologies Poroshell column, 1200 HPLC system, and 6210 Time-of-Flight mass spectrometer for analyzing intact IgG4 antibodies. The method achieves a short retention time of 2.78 minutes with good chromatographic peak shape. Mass spectral analysis successfully resolved various antibody subpopulations, demonstrating routine mass accuracy better than 25 ppm.
Agilent 6210 TOF MS specifications
| Retention time for intact IgG4 | 2.78 min |
|---|---|
| Routine mass accuracy | better than 25 ppm |
| Total analysis time | 9-minute |
| HPLC flow rate | ~500 l/min |
| Column oven temperature | 90°C |
| Capillary voltage (TOF MS) | 5500 V |
| Drying gas temperature | 300°C |
Source: Agilent Antibody Analysis by ESI-TOF LC/MS Application Note
All Agilent 6210 TOF MS documents by type
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the routine mass accuracy demonstrated by the system?
The routine mass accuracy demonstrated by the TOF MS was better than 25 ppm.
From: Agilent Antibody Analysis by ESI-TOF LC/MS Application Note
Which column is used for the optimal chromatographic peak shape?
The Poroshell C8 column was used, resulting in very good chromatographic peak shape.
From: Agilent Antibody Analysis by ESI-TOF LC/MS Application Note
What is the maximum number of samples this system can analyze per day?
The high-throughput LC/MS TOF system is capable of analyzing more than 1000 samples per day.
From: Agilent 6210 TOF MS Application Note for High Throughput LC/MS Analysis of Drug Degradation Products
What flow rate was used for the gradient and column regeneration steps?
The method utilized a flow rate of 1 mL/min for both the gradient runs and the column regeneration process.
From: Agilent 6210 TOF MS Application Note for High Throughput LC/MS Analysis of Drug Degradation Products
What is the maximum temperature applied in the experiment?
A maximum temperature of 80 °C was applied to reduce solvent viscosity.
From: Agilent 1200 Series LC and 6210 TOF MS Application Note
What is the maximum flow rate tolerated by the standard ESI source?
The standard ESI source specifies a maximum flow rate of up to 1 mL/min.
From: Agilent 1200 Series LC and 6210 TOF MS Application Note