Key facts
- Brand
- Agilent
- Model
- Zorbax SPE C18
- Documents
- 5
- Languages
- EN
- Last added
- 2012-07-03
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Agilent Zorbax SPE C18 Cartridge Application Brief for Naproxen Analysis
The document details an analytical procedure for determining levels of naproxen, a pharmaceutical compound used for pain relief, in blood serum. The primary challenge addressed is the interference caused by high protein content in the sample matrix.
To overcome this, the method utilizes a Zorbax SPE C18 cartridge to significantly reduce interfering substances before quantitation via reversed-phase HPLC. The text provides specific instructions for sample preparation, including pretreatment and washing steps, as well as details on the internal standard used (nitrobenzene) and the final HPLC conditions.
Agilent Zorbax SPE C18 specifications
| Cartridge Capacity | 100 mg, 1 ml |
|---|---|
| Part Number | 5184-3590 |
| Column Type | Zorbax SB-C8, 4.6 mmx 150 mm, 3.5 pm |
| Mobile Phase Composition | 50% Acetonitrile in 25 mM Ammonium acetate |
| Detection Wavelength | 240 nm |
| Flow Rate | 1.0 mL/min. |
Source: Agilent Zorbax SPE C18 Cartridge Application Brief for Naproxen Analysis
All Agilent Zorbax SPE C18 documents by type
How to use the product for one specific task, with worked examples.
- Agilent Zorbax SPE C18 Cartridge Application Brief for Naproxen Analysis 2 p
- Agilent Zorbax SPE C18 Cartridge Application Brief for Quinine Analysis 2 p
- Agilent Zorbax SPE C18 Cartridge Application Brief 2 p
- Agilent Zorbax SPE C18 Cartridge Application Brief for Testosterone Analysis 2 p
- Agilent Zorbax SPE C18 EC Cartridge Application Note for Clozapine Analysis 2 p
Questions answered in these manuals
What is the purpose of using nitrobenzene in this analysis?
Nitrobenzene is used as an internal standard for High Load and Low Load samples to ensure accurate quantitation.
From: Agilent Zorbax SPE C18 Cartridge Application Brief for Naproxen Analysis
How does the Zorbax SPE C18 cartridge improve naproxen detection?
It significantly reduces levels of interfering substances, such as blood proteins, allowing the analyte to be clearly detected and quantifiable.
From: Agilent Zorbax SPE C18 Cartridge Application Brief for Naproxen Analysis
What are the volumes and solutions required for conditioning, loading, washing, and eluting the cartridge?
The procedure requires adding 1.0 mL of methanol and 1.0 mL of deionized water to condition the cartridge, followed by loading 1.0 mL of sample, washing with 1.0 mL of deionized water and 1.0 mL of 10% methanol, and finally eluting with 1.0 mL of 1% ammonia in methanol.
From: Agilent Zorbax SPE C18 Cartridge Application Brief for Quinine Analysis
What sample preparation steps are needed to create the high load and low load solutions?
To prepare a high load solution, mix 1 mg/mL quinine with 0.5 mL bovine serum and 0.1 mL deionized water; for a low load solution, use 0.05 mg/mL quinine mixed with the same volumes of serum and water.
From: Agilent Zorbax SPE C18 Cartridge Application Brief for Quinine Analysis
What is the purpose of using a Zorbax SPE C18 cartridge for ranitidine analysis?
It significantly reduces levels of interfering substances, such as blood proteins, allowing the analyte to be easily quantitated.
What internal standard is used in the described method?
Prednisolone is included in the samples as an internal standard.