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Agilent Total RNA Isolation Mini Kit (5185-6000) Protocol
This document provides detailed protocols for isolating total RNA from various biological samples using the Agilent Total RNA Isolation Mini Kit. The kit uses a phenol-free, spin-column technique to produce concentrated, highly purified RNA with minimal genomic DNA contamination. It includes components for animal tissues and cultured cells, and also features new protocols for fibrous tissues, bacteria, and yeast. The manual covers preparation, reagent storage, isolation steps, quality analysis, and troubleshooting. RNA is suitable for downstream applications such as cDNA synthesis, RT-PCR, microarray analysis, and Northern blotting.
Read onlineAgilent Total RNA Isolation Mini Kit (5185-6000) specifications
| Product Number (RNA Kit) | 5185-6000 |
|---|---|
| Product Number (DNase Kit) | 5188-2735 |
| Tissue Weight (Thymus/Spleen) | 15 mg |
| Tissue Weight (Pancreas) | 10 mg |
| Target Gene | mouse GAPDH gene |
| Fragment Size | 79 bp |
Source: Agilent Total RNA Isolation Mini Kit Application Note
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How to use the product for one specific task, with worked examples.
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
How much does the Agilent kit reduce gDNA contamination compared to silica methods?
The Agilent method results in up to 1000-fold lower levels of gDNA contamination compared to a silica column-based method.
What is the lowest level of gDNA contamination achieved in the study?
The lowest level of gDNA contamination achieved was 0.0003% for pancreas tissue when using the Agilent kit with the DNase Accessory Kit.
Does this kit remove genomic DNA?
Yes, a unique mini-prefiltration column removes genomic DNA, typically reducing its level 100- to 1000-fold compared to silica or glass-fiber methods.
From: Agilent Total RNA Isolation Mini Kit (5185-6000) Protocol
What is the minimum volume for RNA elution?
RNA can be eluted with as low as 10 µL of water.
From: Agilent Total RNA Isolation Mini Kit (5185-6000) Protocol