Key facts
- Brand
- Agilent
- Model
- C18-A
- Industry
- Industrial
- Documents
- 3
- Languages
- EN
- Last added
- 2012-06-12
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Agilent Application Note 1926 Separation of Anti-inflammatory and Analgesic Drugs on Polaris C18-A
This document is an application note detailing the high-performance liquid chromatography (HPLC) separation of a specific group of acidic drugs, including anti-inflammatory and analgesic agents. The procedure utilizes an Agilent Polaris C18-A column with dimensions of 150 x 4.6mm.
The method employs a gradient elution starting at 45% and increasing to 80% of solvent B (acetonitrile with 0.1% formic acid) over 10 minutes, with solvent A being water with 0.1% formic acid. The flow rate is set to 1.0 ml/min at ambient temperature, with detection performed using UV at 220 nm. The specific analytes listed for separation include Acetylsalicylic acid, Carbamazepine, Salicylic acid, Tolmetin, Indomethacin, Naproxen, Diclofenac, and Louprofen.
Agilent C18-A specifications
| Column | Polaris C18-A 5y 150 x 4.6mm |
|---|---|
| Column Part Number | A2000150X046 |
| Eluent A | H2O + 0.1% HCOOH |
| Eluent B | MeCN + 0.1% HCOOH |
| Gradient | 45 - 80%B in 10 min |
| Flow Rate | 1.0 ml/min |
| Temperature | ambient |
| Detection | UV @ 220 nm |
Source: Agilent Application Note 1926 Separation of Anti-inflammatory and Analgesic Drugs on Polaris C18-A
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How to use the product for one specific task, with worked examples.
Questions answered in these manuals
Which drugs are separated in this application note?
The note lists Acetylsalicylic acid, Carbamazepine, Salicylic acid, Tolmetin, Indomethacin, Naproxen, Diclofenac, and Louprofen.
From: Agilent Application Note 1926 Separation of Anti-inflammatory and Analgesic Drugs on Polaris C18-A
What is the gradient program for this separation?
The gradient increases from 45% to 80% of solvent B over a period of 10 minutes.
From: Agilent Application Note 1926 Separation of Anti-inflammatory and Analgesic Drugs on Polaris C18-A
What is the stated length and diameter of the Polaris C18-A column?
The column has a length of 150 mm and a diameter of 2.0 mm.
From: Agilent Polaris C18-A Column Application Note: Separation of Fat Soluble Vitamins
What is the specified flow rate for the separation procedure?
The flow rate is set to 0.3 ml/min.
From: Agilent Polaris C18-A Column Application Note: Separation of Fat Soluble Vitamins
How long does the HPLC separation of ten nucleotides take using this column?
An HPLC separation of ten nucleotides was achieved in 24 minutes.
From: Agilent Polaris C18-A Column Application Note for Nucleotide Analysis
What is the flow rate used for the nucleotide analysis procedure?
The flow rate specified for this analysis is 1.5 mL/min.
From: Agilent Polaris C18-A Column Application Note for Nucleotide Analysis