This Application Note demonstrates how a daily throughput of far more than 1000 samples can be achieved together with full spectral data acquisition and accurate mass information with close to FT-MS mass accuracy using the Agilent 1200 Series Rapid Resolution LC system and the Agilent 6210 TOF MS.
This document describes a method for achieving high throughput in LC/MS analyses without compromising data quality. It details the setup of an Agilent 1200 Series Rapid Resolution LC system coupled with an Agilent 6210 Time-of-Flight (TOF) mass spectrometer. The system utilizes alternating column regeneration (ACR) and low delay volume configurations to enable short analysis times with high chromatographic resolution.
| Column ID | 2.1 mm |
|---|---|
| Column length | 50 mm |
| Capillary ID (injection valve) | 0.12 mm |
| DAD acquisition rate | 80 Hz |
| TOF MS acquisition rate | 40 Hz |
| Maximum temperature | 80 °C |
| Flow rate | 1.8 mL/min |
| Capillary voltage | 3000 V |
| Fragmentor voltage | 180 V |
| Skimmer voltage | 40 V |
| Gas temperature | 350 °C |
A maximum temperature of 80 °C was applied to reduce solvent viscosity.
The standard ESI source specifies a maximum flow rate of up to 1 mL/min.
The solution is to intentionally desensitize the TOF MS by altering MS parameters from run to run.