This Application Note describes the use of a high-throughput rapid resolution LC/MS TOF system for detection of impurities in a pharmaceutical formulation in more than 1000 samples per day. The instrument setup and a method for alternating column regeneration in a high-throughput LC system to reduce the overall analysis time by 50 % are also described.
This document details a high-throughput method for detecting and identifying impurities in pharmaceutical formulations, specifically analyzing more than 1000 samples per day. The methodology utilizes an Agilent 1200 Series Rapid Resolution LC system coupled with an Agilent 6210 Time-of-Flight mass spectrometer.
The high-throughput LC/MS TOF system is capable of analyzing more than 1000 samples per day.
The method utilized a flow rate of 1 mL/min for both the gradient runs and the column regeneration process.