This document is an operating manual for a device called Agilent Total RNA Isolation Mini Kit, which can be used for the isolation of fibrous tissue, bacteria and yeast RNA, and is only for research use.
This document provides detailed protocols for isolating total RNA from various biological samples using the Agilent Total RNA Isolation Mini Kit. The kit uses a phenol-free, spin-column technique to produce concentrated, highly purified RNA with minimal genomic DNA contamination. It includes components for animal tissues and cultured cells, and also features new protocols for fibrous tissues, bacteria, and yeast. The manual covers preparation, reagent storage, isolation steps, quality analysis, and troubleshooting. RNA is suitable for downstream applications such as cDNA synthesis, RT-PCR, microarray analysis, and Northern blotting.
| Product Number | 5185-6000 |
|---|---|
| Kit Components | Lysis Solution, Wash Solution, Nuclease-free water, Mini-isolation columns, Mini-prefiltration columns, RNase-free collection tubes |
| Storage Condition | Room temperature |
| RNA Yield | Typical yields vary by sample type (e.g., 0.8 µg/mg tissue for brain, 45 µg/mg for liver) |
Yes, a unique mini-prefiltration column removes genomic DNA, typically reducing its level 100- to 1000-fold compared to silica or glass-fiber methods.
RNA can be eluted with as low as 10 µL of water.