A sensitive and selective analytical method for the determination of aflatoxin G1, G2, B1, and B2 residues in cereals using the Agilent G6410AA LC/MS Triple Quadrupole Mass Spectrometer was developed. This method uses simple sample preparation methods followed by LC/MS/MS. The limits of detection for all aflatoxins were less than 1 ng/mL in cereals.
This document details a sensitive and selective analytical method for determining aflatoxin G1, G2, B1, and B2 residues in cereals. The method utilizes the Agilent G6410AA LC/MS Triple Quadrupole Mass Spectrometer following simple sample preparation steps.
The study optimized Liquid Chromatography (LC) and Mass Spectrometry (MS) conditions, including the use of a ZORBAX Extend C18 column and specific MRM transitions. The developed method achieved limits of detection less than 1 ng/mL for all analyzed aflatoxins.
| Instrument | Agilent G6410 Triple Quadrupole Mass Spectrometer |
|---|---|
| Column | ZORBAX Extend C18, 100 mm x 2.1 mm, 1.8 um |
| Column temperature | 40°C |
| Mobile phase A | 10mM ammonium acetate in water |
| Mobile phase B | Methanol |
| Flow rate | 0.2 mL/min |
| Source | Positive ESI |
| Drying gas flow | 10 L/min |
| Veap | 4000 V |
| Limit of detection (LOD) | Less than 1 ng/mL |
The method determines aflatoxin G1, G2, B1, and B2 residues in cereals.
The limits of detection for all aflatoxins were less than 1 ng/mL in cereals.
A ZORBAX Extend C18 column with dimensions 100 mm x 2.1 mm and 1.8 um particle size is used.