A fast, sensitive, and validated technique for confirming the presence of amphetamine drugs in whole blood using the Agilent G6410A Triple Quadrupole Mass Spec-trometer (QQQ) is presented. Excellent linearity is demon- strated over the range of approximately 15 to 1,000 ng/mL. The amphetamine drugs analyzed in this work include amphetamine, methamphetamine, methyl-enedioxyamphetamine (MDA), and methylenedioxymeth-amphetamine (MDMA) in blood. The drugs have been prepared using an extractive alkylation technique. The sample preparation is then followed by reverse-phase LC/MS/MS using a 1.8-µm particle size C18 column for high chromatographic resolution with a high-speed sepa-ration. As a result, elution times for both analytes and internal standards are all less than or equal to 3.6 min- utes.
This document presents a fast, sensitive, and validated technique for confirming the presence of amphetamine drugs in whole blood. The method utilizes an extractive alkylation technique followed by reverse-phase LC/MS/MS using the Agilent G6410A Triple Quadrupole Mass Spectrometer with electrospray ionization.
The analysis covers amphetamine, methamphetamine, MDA, and MDMA in a concentration range of approximately 15 to 1,000 ng/mL. The method demonstrates excellent linearity (R² > 0.999) with elution times for analytes and internal standards less than or equal to 3.6 minutes.
| Analyte Range | 15 to 1,000 ng/mL |
|---|---|
| Elution Time | <= 3.6 minutes |
| Column Temperature | 60°C |
| Flow Rate | 0.7 mL/min |
| Injection Volume | 2uL |
| Ionization Mode | Positive ESI |
The method analyzes amphetamine, methamphetamine, methylenedioxyamphetamine (MDA), and methylenedioxymethamphetamine (MDMA).
The overall ion ratio of the qualifier to the quantifier ions must fall within a +/- 20% tolerance.
System control and data analysis are provided by Agilent MassHunter B.01.01 software.