This document describes a method for detecting chloramphenicol in honey, shrimp, and chicken using the Agilent G6410AA Triple Quadrupole Mass Spectrometer (QQQ). The sensitivity obtained exceeds the minimum required performance level (MRPL) established by the European Union regulation for food monitoring programs. Using a deuterated internal standard and one simple sample solid phase extraction (SPE) procedure can provide a limit of detection at 10 ppt in sample matrix. The analytical performance of the method was evaluated for three different matrixes and the results show little or no matrix effects. Linearity of response over 2 orders of magnitude was demonstrated (r > 0.99). In addition, good reproducibility of the two required product ion ratios was obtained to meet the EU identification points needed for confirmation. This study is a valuable indicator of the ability of the QQQ for routine quantitative trace analysis of chloramphenicol in honey, shrimp, and chicken.
The document details an analytical method developed to detect, confirm, and quantify chloramphenicol (CAP) residues in food matrices including honey, shrimp, and chicken. The study utilizes the Agilent G6410AA Triple Quadrupole Mass Spectrometer coupled with a solid phase extraction (SPE) procedure and a deuterated internal standard.
The method provides a limit of detection at 10 ppt in the sample matrix.
The flow rate for the HPLC system is set to 0.4 mL/min.
The solvent is evaporated under a nitrogen stream at 40 °C.