Agilent ZORBAX GF-250 Separation of Bio-Rad Gel-Filtration Standards Application Note

About this manual

This document describes the application of size-exclusion chromatography to separate molecules based on their hydrodynamic volume. It details a specific method for calibrating and testing ZORBAX GF-250 or GF-450 columns using Bio-Rad gel-filtration markers.

The procedure utilizes a 9.4 x 250 mm ZORBAX GF-250 column with 200mM Sodium Phosphate mobile phase at pH 7.0. The method highlights the high efficiency of the porous-silica microspheres, allowing for excellent resolution of five protein standards ranging from 1.35 Kdal to 670 Kdal.

What's inside

• Application
• Conditions
• Highlights

Frequently asked questions

What is the stated column size for the ZORBAX GF-250?

The document specifies a column size of 9.4 x 250 mm.

What is the recommended mobile phase for this separation?

Use 200mM Sodium Phosphate at pH 7.0 as the mobile phase.

What are the stated injection volume and flow rate requirements?

The procedure requires a 5pL injection with a flow rate of 2 mL/min.