This document describes the characteristics and usage of the ZORBAX GF-250 column. The column has a wide separation range and is suitable for separating proteins with different PI and hydrophobicity.
This document details an application for calibrating the ZORBAX BioSeries GF-250 column using a mixture of globular proteins. The separation range for this specific column is stated to be from 4000 to 400,000 Daltons.
The text lists several proteins used in the calibration, including Thyroglobulin, Alcohol Dehydrogenase (ADH), Bovine Serum Albumin (BSA), Myoglobin, Lysozyme, and Insulin A-chain. It notes that a good calibration is achieved for mixtures of proteins with different pI and hydrophobicity.
| Column Dimensions | 9.4 x 250 mm |
|---|---|
| Part Number | 884973-901 |
| Mobile Phase | 0.2 M NaH2PO4, pH 7.0 |
| Detection Wavelength | UV 230 nm |
The stated separation range is from 4,000 to 400,000 Daltons.
The column dimensions are 9.4 x 250 mm.
The procedure uses a mobile phase of 0.2 M NaH,PO,, pH 7.0.