Key facts
- Brand
- Agilent
- Model
- SEC-3
- Industry
- Industrial
- Documents
- 2
- Languages
- EN
- Last added
- 2012-07-04
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Agilent Bio SEC-3 Columns Datasheet
The Agilent Bio SEC-3 columns are designed for high-pressure liquid chromatography (HPLC) systems, utilizing 3 µm silica particles coated with a proprietary hydrophilic layer. These columns are optimized for the size exclusion separation of large biomolecules such as antibodies, proteins, peptides, and oligonucleotides.
Read onlineAgilent SEC-3 specifications
| Particle size | 3 µm |
|---|---|
| Pore sizes | 100 Å, 150 Å, 300 Å |
| pH stability | 2–8.5 |
| Operating temperature limit | 80 °C |
| Operating pressure limit | 137 bar (2,000 psi) |
| Maximum pressure | 240 bar (3,500 psi) |
| Working flow rate (7.8 mm id) | 0.1–1.25 mL/min |
| Working flow rate (4.6 mm id) | 0.1–0.4 mL/min |
All Agilent SEC-3 documents by type
Ratings, dimensions and ordering codes, for selection and design work.
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What biomolecules are suitable for separation with Agilent Bio SEC-3 columns?
These columns are designed for antibodies, proteins, peptides, oligonucleotides, polysaccharides and other large bio-molecules.
What is the recommended pH range for using these columns?
The columns can be used in the range pH 2–8.5 to optimize performance and lifetime.
What system was used for the rEPO separation?
The Agilent 1260 Infinity Bio-inert Quaternary LC system.
From: Agilent Bio SEC-3 Application Note for rEPO Separation
What is the recommended flow rate for intact rEPO separation?
The optimized flow rate was 0.35 mL/min.
From: Agilent Bio SEC-3 Application Note for rEPO Separation