Agilent SB-C18 Manuals

This application note describes a method for analyzing oxycodone and its metabolites (noroxycodone, oxymorphone, and noroxymorphone) in plasma using high-performance liquid chromatography/mass spectrometry (HPLC/MS) and the Agilent ZORBAX Rapid Resolution High Throughput (RRHT) StableBond SB-C18 column for chromatographic separation. The method employs an ammonium acetate/acetonitrile gradient and detection by electrospray positive ionization mode mass spectrometry. Spiked human plasma samples are subjected to solid-phase extraction prior to HPLC/MS analysis. The method provides fast, efficient analysis with minimal solvent consumption, which increases productivity, and good linearity (R2 > 0.9900) and reproducibility (less than 10% difference between replicate analyses) for all compounds.

The Agilent ZORBAX SB-C18 RRHT Threaded Column is designed for high-pressure operation (up to 600 bar) and is packed with a high-performance microparticulate C18 packing for high-speed reversed phase HPLC. The StableBond packing is made by chemically bonding a sterically-protected C18 stationary phase to a specially prepared, high purity ZORBAX porous silica microsphere. The special ZORBAX silica support is designed to reduce or eliminate strong adsorption of basic compounds. The densely covered, sterically protected, diisobutyl n-octadecylsilane stationary phase is chemically stable and gives longer column life. As a result, ZORBAX SB-C18 is a stable, reversed-phase packing that can be used for basic, neutral, or acidic samples. It is particularly well suited for use with aggressive mobile phases (for example, pH <2, high ionic strength, ion-pair additives, etc.) since the steric protection of the bonded phase resists degradation caused by such mobile phases. This material has also been shown to be stable when operated at temperatures up to 90 °C. These characteristics are particularly important for use in methods that need long-term stability and reproducibility. ZORBAX SB-C18 is especially suited in applications that use high-sensitivity detectors that require low backgrounds (for example, mass spectrometers). The uniform, spherical, ZORBAX SB-C18 particles have a controlled pore size of 80Å.

This is a product description manual that introduces the features and characteristics of the product, such as the product name, brand, model, etc.

Agilent ZORBAX SB-C8 is a stable, reversed-phase packing that can be used for basic, neutral, or acidic samples. It is particularly well-suited for use with aggressive mobile phases (for example, pH < 2, high ionic strength, ion-pair additives, etc.) since the steric protection of the bonded phase resists degradation caused by such mobile phases. This material has also been shown to be stable when operated at temperatures up to 80 °C. These characteristics are particularly important for use in methods that need long-term stability and reproducibility.

Agilent ZORBAX Rapid Resolution High Definition (RRHD) SB-C18 column is a high-performance particulate C18 packing for high-speed and high resolution reversed-phase HPLC and UHPLC. This column is particularly well-suited for use with aggressive mobile phases (for example, pH < 2, high ionic strength, ion-pair additives, etc.) since the steric protec- tion of the bonded phase resists degradation caused by such mobile phases. This material has also been shown to be stable when operated at temperatures up to 90 °C. These characteristics are particularly important for use in methods that need long-term stability and reproducibility.

ZORBAX SB-C18 is the most rugged, stable commercial C18 column produced by Agilent Technologies.

The document demonstrates the improved liquid chromatography analysis of complex biological fluids using fine particle columns by comparing two columns of different lengths and particle sizes on the analysis of a tryptic digest of human serum albumin (HSA). The columns provide better resolution and comparable sensitivity to 150-mm columns, but in one-sixth the time.