Key facts
- Brand
- Agilent
- Model
- SB-C18
- Industry
- Industrial
- Documents
- 11
- Languages
- JA · EN
- Last added
- 2012-07-06
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Agilent ZORBAX StableBond SB-C18 LC Column Application Note for Oxycodone Analysis
This document details a method for analyzing oxycodone and its oxidation metabolites (noroxycodone, oxymorphone, noroxymorphone) in human plasma. The method combines high-speed liquid chromatography with mass spectrometry detection using an Agilent ZORBAX Rapid Resolution High Throughput StableBond SB-C18 column.
The procedure involves solid-phase extraction of spiked human plasma samples followed by analysis via LC/MS in electrospray positive ion mode. The method achieves good linearity (R² > 0.9900) and reproducibility, with a limit of detection for oxycodone at 0.5 ng/mL. This manual is written in Japanese.
Agilent SB-C18 specifications
| Maximum operating pressure | 600 bar (9000 psi) |
|---|---|
| Maximum operating temperature | 90 °C |
| Particle size | 1.8 um |
| Pore size | 80A |
| Compatible pH range | pH 0.8 to 8.0 |
Source: Agilent ZORBAX SB-C18 RRHT Threaded Column Datasheet
All Agilent SB-C18 documents by type
Ratings, dimensions and ordering codes, for selection and design work.
- Agilent ZORBAX SB-C18 RRHT Threaded Column Datasheet 2 p
- Agilent ZORBAX SB-C18 Rapid Resolution Threaded Column Datasheet
- Agilent ZORBAX SB-C18 Column Datasheet 2 p
- Agilent ZORBAX Rapid Resolution High Definition SB-C18 Threaded Column handbook(1)(1) 2 p
- Agilent ZORBAX Rapid Resolution High Definition SB-C18 Threaded Column handbook(1) 2 p
- Agilent Analysis of Anthocyanins in Common Foods Using an Agilent Poroshell 120 SB-C18 handbook
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the minimum detection limit for oxycodone using this method?
The detection and quantification limit for oxycodone is 0.5 ng/mL.
From: Agilent ZORBAX StableBond SB-C18 LC Column Application Note for Oxycodone Analysis
What are the dimensions of the ZORBAX StableBond SB-C18 column used?
The column measures 2.1 mm in inner diameter and 50 mm in length.
From: Agilent ZORBAX StableBond SB-C18 LC Column Application Note for Oxycodone Analysis
What is the maximum operating pressure for these columns?
The maximum operating pressure is 600 bar (9000 psi).
What is the recommended storage solvent for long term storage?
Long term storage should be in a pure organic solvent, preferably an aprotic liquid such as 100% acetonitrile.
What technology is used in the ZORBAX SB-C18 column?
It uses StableBond C18 packing made by chemically bonding a sterically-protected C18 stationary phase to high purity silica spheres.
What is the particle size of this column?
The column features sub-2-micron particles designed to reduce or eliminate strong adsorption.