This Application Note describes a rapid and sensitive method for quantification of fluvastatin in plasma using the Agilent 1200 Series Rapid Resolution Liquid Chromatography (RRLC) system and the Agilent 6410 Triple Quadrupole LC/MS system. Sample preparation was carried out by a simple protein precipitation step. Atorvastatin at 40 ng/mL was used as the internal standard. The developed method had a short run time of two minutes and was found to be linear in the concentration range of 0.5 ng/mL to 200 ng/mL. The limit of detection (LOD) of the method is 0.2 ng/mL, or 1 pg on-column.
This document details an analytical method developed by Agilent Technologies to quantify fluvastatin in human plasma. The method utilizes the Agilent 6410B Triple Quadrupole LC/MS system coupled with the Agilent 1200 Series Rapid Resolution Liquid Chromatography (RRLC) system. Sample preparation involves a simple protein precipitation step using acetonitrile, followed by centrifugation and reconstitution.
The developed method has a short run time of two minutes.
Fluvastatin and the internal standard were spiked into 250 µL of plasma.
The internal standard concentration was maintained at 40 ng/mL.