The document describes a solid phase extraction method for a pharmaceutical compound called Ranitidine. This compound is used for the treatment and prevention of ulcers and for the relief of heartburn. By using a Zorbax SPE C18 cartridge, levels of interfering substances in the bloodstream can be significantly reduced, allowing for easy quantitation of the analyte of interest using reversed-phase HPLC.
This document presents an application brief for the Zorbax SPE C18 cartridge, demonstrating its effectiveness in reducing matrix interference from blood proteins when analyzing ranitidine. The text illustrates how the cartridge allows for the clear detection and quantitation of the analyte via reversed-phase HPLC.
| Cartridge Capacity | 100 mg |
|---|---|
| Cartridge Volume | 1 mL |
| Column Dimensions | 4.6 mm x 150mm |
| Column Particle Size | 5um |
| Flow Rate | 1.0 mL/min |
| Column Temperature | 35°C |
| Injection Volume | 20 uL |
| Detection Wavelength | 220 nm |
| Recovery Rate | 100% |
It significantly reduces levels of interfering substances, such as blood proteins, allowing the analyte to be easily quantitated.
Prednisolone is included in the samples as an internal standard.
The recovery rate for ranitidine is 100%.