The document describes the significant reduction of matrix compounds in plasma by using a Zorbax SPE C18 cartridge, facilitating the quantification of testosterone-like compounds by reversed-phase HPLC. The document also provides methods for solid phase extraction and sample preparation.
The document details an analytical method for determining levels of testosterone and related compounds in blood serum. It highlights how Solid Phase Extraction (SPE) using a Zorbax SPE C18 cartridge significantly reduces matrix interference from proteins, allowing for clear quantitation by reversed-phase HPLC.
A specific protocol is provided, including sample preparation steps such as loading the sample onto the cartridge and eluting analytes with a methylene chloride/methanol mixture. The document also lists recovery rates for testosterone acetate and propionate under these conditions.
| Cartridge Capacity | 100 mg, 1 mL |
|---|---|
| Column Dimensions | 4.6 mm x 75 mm, 3.5 µm |
| Column Part Number | 966967-906 |
| Recovery of Testosterone Acetate | 95% |
| Recovery of Testosterone Propionate | 92% |
The HPLC method uses a UV detector set to 254 nm.
It is used to reduce levels of matrix compounds in blood samples so that testosterone and related compounds can be quantitated by reversed-phase HPLC.
The procedure requires loading 1.0 mL of sample onto the cartridge.