Agilent Reversed Phase Prep columns demonstrated both good loadability and good scalability for calcium channel blockers using gradient conditions. The columns also dis- played good resolution at all dimensions and allowed a linear scaleup from analytical to preparative dimensions
This document describes a study on the preparative scaleup of calcium channel blockers (Nifedipine, Nimodipine, and Nisoldipine) using Agilent Reversed Phase Prep columns. The authors demonstrate that the columns exhibit good loadability and scalability, allowing for a linear scaleup from analytical to preparative dimensions while maintaining resolution.
The study focuses on the Agilent Prep 5-um Scalar column and the Agilent Prep-C18 series. It outlines the theoretical considerations for scaleup, including the relationship between sample capacity and column dimensions, and presents data on column loadability and scalability across various column internal diameters (4.6 mm to 50.8 mm).
| Column Type | Agilent Prep-C18 |
|---|---|
| Particle Size | 5-um |
| Column Length | 100 mm |
| Detector Signal | 345 nm |
| Sample Concentration | 80 mg/mL |
The study separates Nifedipine, Nimodipine, and Nisoldipine.
The scaleup process should stop when resolution drops below 1.2.
The sample stock solution is prepared in dimethylsulfoxide (DMSO).