Agilent Human 14 Multiple Affinity Removal Spin Cartridge Instruction Guide

About this manual

This document provides step-by-step instructions for using the Agilent Human 14 Multiple Affinity Removal Spin Cartridge to process human serum or plasma samples. It details procedures for dilution, filtration, sample application, centrifugation, elution, and re-equilibration. The guide also includes information on storing the cartridge and analyzing collected fractions.

The document references additional Agilent products for proteomics research, such as LC columns, digestion kits, and cleanup tools, and directs users to Agilent’s website for more information.

What's inside

• Dilute and filter sample
• Remove buffer
• Apply sample and incubate
• Centrifuge and collect fractions
• Elute bound fraction
• Re-equilibrate cartridge
• Analyze Fl + F2
• Store or discard eluate

Frequently asked questions

How do I prepare the sample for processing?

Dilute 8-10 pL human serum/plasma to 200 μL with Buffer A, then filter through a 0.22 μm spin filter.

What is the part number for the Agilent Human 14 Multiple Affinity Removal Spin Cartridge?

The part number is 5188-8560.

What is the centrifuge speed and time for the first step?

Centrifuge 2.5 minutes at 100 x g.

What is the purpose of the spin cartridge?

It is used for multiple affinity removal in serum/plasma processing.