Key facts
- Brand
- Agilent
- Model
- CFX96
- Industry
- Industrial
- Documents
- 3
- Languages
- EN
- Last added
- 2012-07-04
Start here
Stratagene Brilliant III Ultra-Fast SYBR Green QRT-PCR Master Mix Quick Reference Guide for Bio-Rad CFX96
This document is a Quick Reference Guide for the Stratagene Brilliant III Ultra-Fast SYBR Green QRT-PCR Master Mix. It provides an optimized protocol specifically designed for use with the Bio-Rad CFX96 Real-Time PCR Detection System.
The guide details the steps to prepare experimental reactions, including mixing components on ice and adding RNA templates. It also outlines the procedure for setting up the experiment in the CFX Manager software, running the thermal profile, and analyzing the resulting data.
Agilent CFX96 specifications
| Final Reaction Volume | 20 μl |
|---|---|
| Primer Concentration Range | 150–500 nM |
| DTT Quantity per reaction | 0.2 μl of 100 mM DTT |
All Agilent CFX96 documents by type
The shortest path to a working setup, usually only a few pages.
Ratings, dimensions and ordering codes, for selection and design work.
Questions answered in these manuals
How long does it take to achieve qPCR results with this master mix?
The Brilliant III Ultra-Fast QPCR Master Mixes can deliver results in less than 40 minutes on fast cycling real-time PCR systems.
What is the optimal synthesis temperature for the QRT-PCR reagents?
Highly efficient one-step QRT-PCR is performed using a Moloney-based RT for 1st strand synthesis with optimal performance at a synthesis temperature of 50°C.
Which instrument is this guide optimized for?
This guide provides an optimized protocol for using the Stratagene Brilliant III Ultra-Fast SYBR Green QRT-PCR Master Mix with the CFX96 Real-Time PCR Detection System.
What is the suggested RNA quantity range for total RNA?
The table lists a suggested quantity range of 0.1 pg – 100 ng per reaction for Total RNA.