This document describes the application of Agilent's Brilliant III Ultra-Fast QPCR Master Mixes on the BioRad CFX96 Real-Time PCR platform. The product features a novel fast Taq mutant that enables qPCR results in under 40 minutes. The enhanced rapid hot start capability saves time and reduces primer-dimer formation. The optimized fast cycling formulation ensures reliable and reproducible data with shorter run times. The master mixes are compatible with any fluorescent detection chemistry, including both sequence-specific probes and SYBR® Green dyes.
Agilent Technologies presents the Brilliant III Ultra-Fast QPCR Master Mixes, designed to deliver improved real-time quantitative PCR (qPCR) results on the BioRad CFX96 Real-Time PCR platform. These reagents feature a novel fast Taq mutant and an enhanced rapid hot start capability, enabling gene-specific amplification with minimal primer-dimer formation in under 40 minutes.
The product line includes formulations compatible with both sequence-specific probes and SYBR® Green dyes for one-step QRT-PCR or two-step cDNA synthesis. The datasheet highlights superior sensitivity at low target concentrations and improved reproducibility compared to competitor master mixes, supported by data on standard curves and dissociation analysis.
The Brilliant III Ultra-Fast QPCR Master Mixes can deliver results in less than 40 minutes on fast cycling real-time PCR systems.
Highly efficient one-step QRT-PCR is performed using a Moloney-based RT for 1st strand synthesis with optimal performance at a synthesis temperature of 50°C.
The ordering information assumes a reaction volume of 20 µl.