Provides an optimized protocol for using the Stratagene Brilliant III Ultra-Fast SYBR® Green QRT-PCR Master Mix with the CFX96 Real-Time PCR Detection System from Bio-Rad.
This document is a Quick Reference Guide for the Stratagene Brilliant III Ultra-Fast SYBR Green QRT-PCR Master Mix. It provides an optimized protocol specifically designed for use with the Bio-Rad CFX96 Real-Time PCR Detection System.
The guide details the steps to prepare experimental reactions, including mixing components on ice and adding RNA templates. It also outlines the procedure for setting up the experiment in the CFX Manager software, running the thermal profile, and analyzing the resulting data.
| Final Reaction Volume | 20 μl |
|---|---|
| Primer Concentration Range | 150–500 nM |
| DTT Quantity per reaction | 0.2 μl of 100 mM DTT |
This guide provides an optimized protocol for using the Stratagene Brilliant III Ultra-Fast SYBR Green QRT-PCR Master Mix with the CFX96 Real-Time PCR Detection System.
The table lists a suggested quantity range of 0.1 pg – 100 ng per reaction for Total RNA.
The CFX Manager software is used to create a new experiment and edit the protocol parameters.