Agilent 6520 QTOF LC/MS System Metabolite Identification Application Note

This Application Note describes the identification of pharmaceutical metabolites using the Agilent 6520 Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) LC/MS system and Agilent MassHunter Metabolite Identification (MetID) software.

Brand: Agilent
Category: Other
Document type: Application Note
Language: English
Pages: 8
File format: PDF
File size: 1.4 MB
Published: 02 July, 2012
Updated: 30 September, 2023
Model: 6520 Q-TOF
MD5 checksum: 53ACDEE958C21EE8328F3E40A068C94E
PDF: Agilent 6520 QTOF LC/MS System Metabolite Identification Application Note PDF

About this manual

This document details the identification of pharmaceutical metabolites during drug discovery and development. It highlights the benefits of using the Agilent 6520 Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) LC/MS system combined with Agilent MassHunter Metabolite Identification (MetID) software.

What's inside

Identification of pharmaceutical metabolites
Expanded dynamic range
Higher mass resolution
Structure elucidation
Experimental equipment
Sample preparation
High resolution LC/MS method
Data analysis with MassHunter MetID
Results and discussion

Specifications

System Model	Agilent 6520 Accurate-Mass O-TOF LC/MS system
Software	Agilent MassHunter Metabolite Identification software
Column	ZORBAX SB-C18 column, 2.1 x 150 mm, 1.8 pm
Flow rate	0.5 mL/min
Mass range	100-1000
Collision energy	35 V

Frequently asked questions

What is the purpose of this application note?

It describes the identification of pharmaceutical metabolites using the Agilent 6520 Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) LC/MS system and Agilent MassHunter Metabolite Identification (MetID) software.

How is high mass resolution utilized?

High mass resolution is used to elucidate the structures of metabolites by differentiating between fragments of isobaric mass in MS/MS spectra.

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