This document compares the use of micro and regular flow cells with ZORBAX 300SB-C8 (3.5 µm) column for peptide and protein analysis. It highlights the reduction of analysis time without compromising efficiency. By reducing the flow cell size, the effects of extra-column volume are minimized with small particle-size packing.
This document presents a technical comparison between micro flow cells and regular flow cells when used with small particle-size column packings, specifically the ZORBAX 300SB-C8 (3.5 m). The analysis illustrates that using a micro flow cell reduces band broadening and improves peak resolution for proteins and peptides compared to a regular flow cell setup.
| Column Packing | ZORBAX 300SB-C8 (3.5m) |
|---|---|
| Column Dimensions | 4.6 x 50 mm |
| Mobile Phase A | 95:5 Water:ACN with 0.10% TFA |
| Mobile Phase B | 5:95 Water:ACN with 0.085% TFA |
| Gradient | 10-60% B in 10 minutes |
| Injection Volume | 10pL |
| Flow Rate | 1 mL/min |
| Detection | UV( 215 nm) |
A micro flow cell is suggested when using small particle-size column packings.
It reduces band broadening and improves peak resolution for proteins and peptides.
Using a 50-mm column length with ZORBAX 300SB-C8 (3.5 m) can reduce the time of analysis for peptides and proteins without sacrificing efficiency.
Reducing the flow cell size minimizes extra-column volume effects with small particle-size packing.