This document introduces the effect of column aging with acidic mobile phase on ZORBAX 300SB-C8 (4.6 x 150 mm) (Agilent P/N: 883995-906) column.
This document presents an application study titled 'Effect of Column Aging with Acidic Mobile Phase: Silanol-Sensitive Peptide Separation, pH 7'. It evaluates the ZORBAX 300SB-C8 (4.6 x 150 mm) column using a specific mobile phase gradient from 10-100% B over 45 minutes. The conditions include 10mM NH4H2PO4 at pH 7.0 for solvent A and 50 mM sodium perchlorate in 50% ACN / 50% H2O for solvent B, with a flow rate of 1 mL/min at 40°C.
| Column Dimensions | 4.6 x 150 mm |
|---|---|
| Part Number | 883995-906 |
| Flow Rate | 1 mL/min |
| Temperature | 40°C |
| Detection Wavelength | 214 nm |
| Gradient Duration | 45 min |
No, the silanol content does not change even after 18 liters of low-pH eluent.
It serves as a very sensitive neutral-pH test of silanol accessibility.