This document introduces the characteristics of ZORBAX 300SB-C8 and 300SB-CN two chromatographic columns from Agilent Technologies, which can achieve stable separation of high molecular weight, alkylated, wheat glutenin protein subunits.
This application note presents a chromatographic comparison between the ZORBAX 300 SB-C8 and ZORBAX 300 SB-CN stationary phases. The analysis focuses on the separation of high-molecular-weight, alkylated wheat glutenin protein subunits, specifically o-gliadins.
The document highlights that improved separations are achieved by utilizing the different bonded-phase chemistries of the two columns. It notes that the sterically protected SB bonded phases are hydrolytically stable, particularly at low pH, ensuring stable and reproducible results.
| Column Dimensions | 4.6 x 150 mm |
|---|---|
| Gradient Duration | 60 min. |
| Flow Rate | 1.0 mL/min. |
| Mobile Phase Additive | 0.1% TFA |
The manual specifies that both column types have dimensions of 4.6 x 150 mm.
The stated procedure uses a mobile phase flow rate of 1.0 mL/min.