This application note demonstrates the advantages of using high temperature and low pH conditions with ZORBAX StableBond C8 packing materials for high-efficiency preparative separations.
This document describes a successful application for the preparative separation of crude synthetic amyloid B(1-42) peptide. The procedure utilizes high temperature conditions to achieve good peak shape and excellent recoveries exceeding 70%, whereas separations at lower temperatures yield very poor results with less than 10% recovery.
The method highlights the stability of ZORBAX StableBond C8 packing materials under extreme conditions, including high temperatures up to 80°C and low pH. The highly efficient 21 mm ID column packed bed remains stable even at these elevated temperatures.
The separation is performed at a temperature of 80°C.
The flow rate is set to 15mL / min.