Agilent's Poroshell 300 reversed-phase HPLC columns offer higher resolution and flow rates for faster and more efficient separation of peptides, proteins, and antibodies.
This document introduces the Agilent Poroshell 300 reversed-phase HPLC columns, designed to provide faster and more efficient separations for peptides, proteins, and antibodies in drug development and clinical trials. The technology utilizes a unique superficially porous particle design that allows large molecules to diffuse quickly in and out of the particle pore structure, resulting in improved efficiency and decreased peak width at higher flow rates compared to totally porous columns.
The Poroshell 300 line includes various bonded phases such as 300SB-C18, 300SB-C8, 300SB-C3, and 300Extend-C18. These columns offer robust performance at temperatures up to 90°C and pH ranges from 2 to 11.5. The document highlights significant time savings, noting that analysis time can be reduced by up to 90% when adapting wide-pore reversed-phase methods to the Poroshell 300 format.
| Particle Design | Superficially porous particle with a solid core and thin porous layer |
|---|---|
| Pore Diameter | 300 Angstroms |
| Column Dimensions | 2.1 x 75 mm |
| Maximum Temperature | 90°C |
| pH Range | 2 to 11.5 |
| Analysis Time Reduction | Up to 90% |
It allows large molecules to quickly diffuse in and out of the particle pore structure, resulting in improved efficiency and decreased peak width at higher flow rates.
Analysis time can be reduced by up to 90% when adapting wide-pore reversed-phase methods to the Poroshell 300 format.
The line is offered in three bonded phases: 300SB-C18, 300SB-C8, and 300SB-C3.