This paper introduces a method for separating proteins using ZORBAX Poroshell 300SB columns under low pH conditions at high temperatures. The method uses a linear gradient elution method with a flow rate of 1.0 mL/min and a column temperature of 40 °C to 75 °C. The results show that the resolution of the peaks is significantly improved as the column temperature is increased.
This document describes an HPLC method that utilizes elevated temperatures to increase separation selectivity and resolution for complex peptide and protein mixtures. The study focuses on the stability of ZORBAX Poroshell 300SB columns at high temperatures, allowing them to be used effectively up to 90 °C.
| Maximum Operating Temperature | 90 °C at low pH |
|---|---|
| Particle Type | Superficially porous particles |
The columns are stable and can be used at temperatures up to 90 °C.
Elevating the column temperature can effectively increase separation selectivity and resolution for ionizable compounds like proteins.