Poroshell 300SB-C18 is a tool for fast, high-resolution protein separations. It consists of a thin layer of porous silica on a solid core, allowing for protein separation in minutes or seconds with high resolution. Bonded with the patented StableBond C18 phase, it provides reliable separations and unmatched column stability. It can also separate at high temperatures with minimal bleed, resulting in clean MS signals at ultra-high speed. Poroshell 300SB also has low backpressure, enabling separation at very high flow rates. This is a tool to revolutionize proteomics and protein analysis.
The Agilent Poroshell 300SB-C18 is a revolutionary chromatography media constructed of a thin layer of porous silica on a solid core. This unique particle design provides rapid mass transfer, enabling very fast, high-resolution reversed-phase HPLC separation of proteins, DNA, and other macromolecules.
The Poroshell 300SB-C18 is an analytical column with dimensions of 2.1 x 75 mm and uses a 5 µm particle.
The default loop wash is 5X the injection volume.
The temperature used in the high-throughput protein analysis example is 70°C.