Agilent Brilliant III Ultra-Fast QPCR/QRT-PCR Master Mixes for Mx3005P/Mx3000P handbook

Brilliant III Ultra-Fast QPCR/QRT-PCR Master Mixes are Agilent's next generation universal quantitative PCR reagents that are suitable for fast or standard cycling conditions on virtually any real-time PCR system.


Agilent Brilliant III Ultra-Fast QPCR/QRT-PCR Master Mixes for Mx3005P/Mx3000P handbook - cover page
Brand
Agilent
Category
Other
Document type
Datasheet
Language
English
Pages
2
File format
PDF
File size
359 KB
Published
04 July, 2012
Updated
30 September, 2023
MD5 checksum
F07C6AC7D11A903D820980B83BB064E4

About this manual

The Agilent Brilliant III Ultra-Fast QPCR and QRT-PCR Master Mixes represent the next generation of universal quantitative PCR reagents suitable for fast or standard cycling conditions on virtually any real-time PCR system. These reagents feature a newly engineered Taq derived mutant delivering faster extension rates combined with an optimized buffer formulation and novel hot-start technology to minimize non-specific amplification products.

What's inside

  • Novel fast Taq mutant
  • Enhanced hot start capability
  • Optimized fast cycling formulation
  • Improved Sensitivity of Detection
  • Low Copy Discrimination
  • Ordering Information
  • SYBR Green QPCR Master Mixes
  • AffinityScript QPCR cDNA Synthesis Kit

Specifications

Reaction Timeunder 50 minutes
CompatibilityMx3000P and Mx3005P
Amplification Efficiency96.4%
Standard Curve R20.999
RT Synthesis Temperature50°C
Packaging Qty2 x 2 ml

Frequently asked questions

What is the maximum reaction time for Brilliant III Ultra-Fast reagents?

The reagents allow the completion of real-time experiments in less than 50 minutes on the Mx platform.

Which Agilent instruments are validated for these reagents?

The reagents have been validated on the Agilent Mx3000P and Mx3005P instruments.

What detection chemistries are compatible with these master mixes?

They are compatible with both sequence specific probes and SYBR® Green dyes.

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