This application note describes how to use RapidFire LC/MS/MS to rapidly detect acrylamide in food with high sensitivity.
The document presents an analytical method to detect acrylamide, a potential carcinogen found in cooked foods, utilizing liquid chromatography-tandem mass spectrometry (LC/MS/MS). The procedure is designed for high throughput, capable of analyzing up to 96 samples including standards within approximately five hours.
The method employs a Varian 1200L LC/MS/MS system equipped with an ESI source and a CTC Analytics HTS PAL Autosampler. Sample preparation involves extraction using internal standards followed by filtration through a Captiva kit. The analysis achieves linear calibration curves in the range of 10,000 to 20 ppb, with limits of detection at 8 ppb and quantification at 20 ppb.
| Limits of Detection (LOD) | 8 ppb |
|---|---|
| Limits of Quantification (LOQ) | 20 ppb |
| Calibration Range | 10,000 to 20 ppb |
| Correlation Coefficient (r) | 0.994 |
| Injection Volume | 10 pL |
| Flow rate | 0.2 mL per min |
The limit of detection is 8 ppb.
The method can analyze any combination of 96 samples in a little more than 5 hours.
The total time for this analysis was approximately 6 hours.