This Application Note describes a method for establishing the dynamic range of the Varian 1200L using TX1360 and its major OH-Metabolite as the model compounds.
The document details an experiment to establish the dynamic range of the Varian 1200L Electrospray (ESI)-LC/MS/MS system. The study utilizes TX1360, a proprietary CNS drug, and its major hydroxylated metabolite (TX1370) as model compounds, with Buspirone serving as the internal standard. The objective was to validate the system's performance for concentration ranges exceeding the saturation limits of older mass spectrometer models.
| Column Dimensions | 50 mm x 1.0 mm |
|---|---|
| Column Type | C-18 |
| Injection Volume | 10 pL |
| Collision Gas | 2.1 mTorr Argon |
| Drying Gas Pressure | 23 psi |
| Nebulizing Gas Pressure | 60 psi |
| Capillary Voltage | -40V |
| Detector Voltage | 2000V |
| Correlation Coefficient | 0.99 |
| Calculated Dynamic Range | 5.0E04 |
The study used TX1360, a proprietary CNS drug, and its major hydroxylated metabolite (TX1370) as the test compounds.
Buspirone was selected as the internal standard for this work.
The calculated dynamic range for the CNS drug is equal to 5.0E04.
The correlation coefficient for both TX1360 and TX1370 calibration curves was 0.99.