Key facts
- Brand
- Agilent
- Model
- GF-250
- Industry
- Industrial
- Documents
- 8
- Languages
- EN
- Last added
- 2012-07-04
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Agilent Zorbax PrepHT GF-250 and GF-450 Datasheet
The document describes the Agilent Zorbax PrepHT GF-250 and GF-450 as surface-stabilized, hydrophilic gel filtration columns designed for the size separation of biological macromolecules. These columns offer superior efficiency and pH stability when used with aqueous buffer solutions in the pH range of 3.0 to 8.5.
Read onlineAgilent GF-250 specifications
| Optimal Separation Range (GF-450) | 900,000 to 25,000 Daltons |
|---|---|
| Optimal Separation Range (GF-250) | 250,000 to 10,000 D |
| Recommended pH Limits | pH 3 to 8.5 |
| Maximum Operating Temperature | 40°C |
| Protein Recovery | 85-100% |
All Agilent GF-250 documents by type
Ratings, dimensions and ordering codes, for selection and design work.
How to use the product for one specific task, with worked examples.
- Agilent ZORBAX GF-250 Separation of Bio-Rad Gel-Filtration Standards Application Note 2 p
- Agilent ZORBAX GF-250 Column Calibration Using Bio-Rad Gel-Filtration Standards Application Note 2 p
- Agilent ZORBAX GF-250 SEC Application Note 2 p
- Agilent ZORBAX GF-250 Column Calibration with Globular Proteins Application Note 2 p
Questions answered in these manuals
What is the recommended pH range for these columns?
The recommended pH limits for these columns are pH 3 to 8.5.
Can the columns be used with organic solvents?
Yes, the GF-250 and GF-450 columns are compatible with most organic solvents.
What is the recommended flow rate for GF-250 columns?
Recommended flow rates are from 0.5-3.0 mL/min, typically 1.0 mL/min.
Can GF-250 columns be used at high pH?
Yes, the columns may be used for short periods at pH 8.5 due to zirconia stabilization.
What is the purpose of using denaturing mobile phases in SEC?
Denaturing mobile phases containing guanidine hydrochloride or SDS are used to insure un-aggregated, monomers for proper size determination.
From: Agilent ZORBAX GF-250 Application Note: Comparison of Denaturing and non-Denaturing Mobile Phases
How does denaturation affect the separation results?
Denaturation shifts molecules toward larger apparent size and smaller retention volume.
From: Agilent ZORBAX GF-250 Application Note: Comparison of Denaturing and non-Denaturing Mobile Phases