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The Agilent Zorbax Bio Series GF-250 is a surface-stabilized, hydrophilic gel-filtration column designed for the size separation of water-soluble macromolecules, particularly proteins. It utilizes wide-pore, small particle-diameter silica support rendered hydrophilic by covalently bonding an organosilane reagent to produce a homogeneous monolayer surface coverage.
This column is specifically engineered to provide superior efficiency, pH stability, and operational lifetime when using typical aqueous buffer solutions with a pH range of 3.0 to 8.5. The zirconium-stabilized particles are highly resistant to base-catalyzed hydrolysis, eliminating the need for extended polymeric coatings often required in other gel-filtration media.
| Molecular Weight Range | 400,000 to 4,000 Daltons (D) |
|---|---|
| Linear Separation Range | 250,000-10,000 D |
| pH Stability Range | 3.0 - 8.5 |
| Particle Diameter | Nominal 4-4.5 µm |
| Surface Area | 140 m²/gram |
| Maximum Flow Rate | 3 mL/min |
| Maximum Temperature | 40°C |
Recommended flow rates are from 0.5-3.0 mL/min, typically 1.0 mL/min.
Yes, the columns may be used for short periods at pH 8.5 due to zirconia stabilization.
Protein recoveries are typically 85-100% of the total material applied to the column.
The column must be flushed with at least 10 column volumes (120 mL) of new buffer.