This Application Note describes the rapid separation of metabolites generated from in-vitro experiments using the Agilent 1290 Infinity LC system. It also explains the fast acquisition of TOF mass spectra using Agilent 6530 Accurate-Mass Quadrupole Time-of-Flight LC/MS systems. The software-assisted identification of main metabolites from in-vitro experiments using Agilent MassHunter Metabolite Identification software is discussed. The generation of reports for the identified metabolites using Agilent MassHunter software is also covered.
This document details a workflow for analyzing the metabolism of pharmaceutical drug candidates. It utilizes the Agilent 1290 Infinity LC system for rapid separation and the Agilent 6530 Accurate-Mass Q-TOF LC/MS system for fast acquisition of mass spectra. The identified metabolites are then analyzed using Agilent MassHunter Metabolite Identification software to generate reports.
| Flow rate | 0.8 mL/min |
|---|---|
| Column temperature | 60 °C |
| Mass range | 100-1000 m/z |
| Acquisition rate | 5 spectra/s |
Agilent MassHunter Metabolite Identification software is used for fast, software-assisted identification of main metabolites.
This document describes a method for the rapid separation and identification of main metabolites generated from pharmaceutical drugs in in-vitro experiments.
The method uses a ZORBAX SB-C18 column with dimensions of 2.1 x 50 mm and a particle size of 1.8 ym.
The incubation was carried out at 37 °C for 60 minutes.