Agilent 300SB-C3 Manuals

Agilent Zorbax 300SB-C3 Reversed-Phase Chromatography Column Datasheet

The Agilent Zorbax 300SB-C3 is a premium-quality reversed-phase chromatography column specifically designed for the separation of biological macromolecules, such as peptides and proteins. The column utilizes a StableBond column packing material prepared by covalently bonding a sterically protected organosilane to ultra-high purity 300A Zorbax silica support. This construction ensures highly efficient and stable chromatography columns with superior stability at low pH and minimal resistance to mass transfer.

The document provides comprehensive operational guidelines, safety considerations, and care instructions for the column. It specifies that the column should be operated between pH 2 and 7 for optimum lifetime, with a maximum operating pressure of 400 bar (6000 psi) and a maximum recommended temperature of 80°C. The text also outlines applications for separating peptides and proteins using gradients of acetonitrile and various acids, along with procedures for column cleaning, frit replacement, and storage.