Key facts
- Brand
- Agilent
- Model
- 300SB-C18
- Industry
- Industrial
- Documents
- 10
- Languages
- EN
- Last added
- 2012-07-05
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Agilent ZORBAX RRHD 300SB-C18 Technical Overview
The document provides a technical overview of the Agilent ZORBAX RRHD 300SB-C18 1.8 µm column, designed for ultra-high-performance liquid chromatography (UHPLC) of proteins and peptides. It highlights the use of StableBond technology to ensure stability under acidic conditions required for protein analysis.
The text details application examples using short 50 mm columns for intact protein separation at various flow rates and temperatures, as well as longer 100 mm columns for peptide digest analysis. Reproducibility data after multiple runs is also presented.
Agilent 300SB-C18 specifications
| Particle Size | 5-um silica particles |
|---|---|
| Shell Thickness | 0.25-1m-thick shell of 300A pores |
| Nitrogen Surface Area | about 4.5 m?/g |
| Maximum Operating Pressure | 400 bar (6000 psi) |
| Max Temp at pH <5 | 70°C |
| Max Temp at pH 5-8 | 40° C |
Source: Agilent Technologies POROSHELL 300SB-C18 HPLC Column Datasheet
All Agilent 300SB-C18 documents by type
Ratings, dimensions and ordering codes, for selection and design work.
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the stated purpose of the Agilent ZORBAX RRHD 300SB-C18 1.8 pm column?
It is a reverse phase media designed for UHPLC analysis of proteins and peptides.
What are the dimensions of the column used for analyzing intact proteins?
The column used for intact protein analysis has dimensions of 2.1 x 50 mm.
What is the maximum operating pressure for this column?
The maximum operating pressure is 400 bar or 6000 psi.
From: Agilent Technologies POROSHELL 300SB-C18 HPLC Column Datasheet
What are the pH limits for using this column?
The column should be avoided below pH 0.9 or above pH 8.0.
From: Agilent Technologies POROSHELL 300SB-C18 HPLC Column Datasheet
What is the particle size of the Poroshell 300SB-C18 column?
The column uses a 5 micron particle.
From: Agilent Poroshell 300SB-C18 RP-HPLC Column Product Leaflet
At what temperature can the column be used for separations?
The column allows separation at temperatures up to 90°C.
From: Agilent Poroshell 300SB-C18 RP-HPLC Column Product Leaflet