Key facts
- Brand
- Agilent
- Model
- 300SB-C8
- Industry
- Industrial
- Documents
- 9
- Languages
- EN
- Last added
- 2012-07-04
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Agilent Zorbax 300SB-C8 HPLC Column Datasheet
The Agilent Zorbax 300SB-C8 is a microparticulate column packing designed specifically for reversed-phase high-performance liquid chromatography (HPLC) of peptides and proteins. It features StableBond packing made by chemically bonding a sterically-protected octyl stationary phase to ultra-high-purity, porous-silica microspheres.
Read onlineAgilent 300SB-C8 specifications
| Particle Pore Size | 300A |
|---|---|
| Maximum Operating Pressure (up to 9.4mm ID) | 400 bar (6000 psi) |
| Maximum Recommended Operating Temperature | 80°C |
| Recommended pH Range | pH 1.0 to 8.0 |
All Agilent 300SB-C8 documents by type
Ratings, dimensions and ordering codes, for selection and design work.
- Agilent ZORBAX 300SB Series Effect of Bonded Phase Type on Peptide Separation 2 p
- Agilent ZORBAX 300SB-C8 Effect of Particle Size on Column Efficiency Technical Note 2 p
- Agilent ZORBAX 300SB-C8 (3.5 m) Micro Flow Cell Comparison Application Note 2 p
- Agilent Technologies Rapid HPLC Analysis of Monoclonal Antibody IgG1 Light Chains Using ZORBAX Poroshell 300SB-C8. 2 p
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the Zorbax 300SB-C8 primarily designed for?
It is designed specifically for reversed-phase HPLC of peptides and proteins.
Is the Zorbax 300SB-C8 suitable for low pH conditions?
Yes, it is particularly well suited for use with aggressive mobile phases at low pH (e.g., pH < 2).
What is the recommended storage solvent for these columns?
Long-term storage should be in a pure organic solvent, preferably an aprotic liquid such as 100% acetonitrile.
From: Agilent ZORBAX RRHD 300SB-C8 Threaded Column Datasheet
Are these columns suitable for use with mass spectrometers?
Yes, they are especially suited for applications using high-sensitivity detectors that require low backgrounds, such as mass spectrometers.
From: Agilent ZORBAX RRHD 300SB-C8 Threaded Column Datasheet
What is the effect of changing the bonded phase on peptide separation?
Significant differences in selectivity can be achieved by changing the ZORBAX StableBond phase.
From: Agilent ZORBAX 300SB Series Effect of Bonded Phase Type on Peptide Separation
What are the part numbers for the ZORBAX columns tested?
The part numbers are 883995-902, 883995-906, and 883995-905.
From: Agilent ZORBAX 300SB Series Effect of Bonded Phase Type on Peptide Separation