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- Brand
- Agilent
- Model
- 390-MDS
- Industry
- Industrial
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- 3
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- EN
- Last added
- 2012-06-12
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Analysis of Polysaccharides by GPC Viscometry using the Agilent 390-MDS Multi Detector Suite Application Note
Polysaccharides are complex polymers where structural differences, such as branching, significantly influence properties like solution viscosity. This document details an analysis of two specific samples: pullulan, which has a linear structure, and dextran, which is highly branched.
The study utilizes Gel Permeation Chromatography (GPC) viscometry employing the Agilent 390-MDS Multi Detector Suite. This system combines a viscometer with a differential refractive index detector to accurately determine molecular weights and investigate solution viscosity via the Universal Calibration approach.
Agilent 390-MDS specifications
| Columns | 3x Agilent PLgel Olexis, 300 x 7.5mm |
|---|---|
| Eluent | DMSO/DMAc (4:1) (0.1% LiBr) |
| Flow Rate | 1.0 mL/min |
| Column Temperature | 60 °C |
| Detector Temperature | 60 °C |
Source: Agilent 390-MDS Analysis of Starches by Gel Permeation Chromatography with Viscometry
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Questions answered in these manuals
What polysaccharide samples are analyzed in this application note?
The document describes the analysis of pullulan, which has a linear structure, and dextran, which is highly branched.
What are the dimensions of the columns used for the analysis?
The procedure uses 2x Agilent PL aquagel-OH MIXED-M columns that measure 300 x 7.5 mm.
What technique is used to determine accurate molecular weights for biopolymers like starches?
Gel permeation chromatography (GPC) with Universal Calibration, employing a viscometer in combination with a differential refractive index detector.
From: Agilent 390-MDS Analysis of Starches by Gel Permeation Chromatography with Viscometry
What caused the different thickening properties of the two analyzed starch samples?
The samples showed stark differences in molecular weight distributions, with one having a bi-modal distribution, and structural differences indicated by Mark-Houwink plots.
From: Agilent 390-MDS Analysis of Starches by Gel Permeation Chromatography with Viscometry
What solvent is recommended for analyzing PDMS with the 390-MDS?
Toluene is preferable because PDMS is isorefractive with tetrahydrofuran, making THF unsuitable for analysis.
From: Agilent 390-MDS Multi Detector Suite Application Note for PDMS Analysis by GPC Viscometry
What are the specified flow rate and injection volume conditions?
The method requires a flow rate of 1.0 mL/min and an injection volume of 100 pL.
From: Agilent 390-MDS Multi Detector Suite Application Note for PDMS Analysis by GPC Viscometry