Agilent ProSEC 300S Columns for Protein SEC Data Sheet

Agilent ProSEC 300S columns for protein SEC eluent selection and flow rate

Brand: Agilent
Category: Other
Document type: Datasheet
Language: English
Pages: 2
File format: PDF
File size: 443 KB
Published: 04 July, 2012
Updated: 30 September, 2023
Model: ProSEC 300S
MD5 checksum: F01C6C6FE3995A2360F8864E35CCA607
PDF: Agilent ProSEC 300S Columns for Protein SEC Data Sheet PDF

About this manual

The document details the ProSEC 300S columns, which contain hydrophobic silica particles designed for the separation of biomolecules including proteins and peptides. It covers installation requirements, such as the use of specific stainless steel tubing and compatible connectors to minimize dead volume. The text also specifies that column connections must be made using Parker compatible 1/16 in nuts and ferrules.

What's inside

Installation
Column connection
Eluent Selection and Flow Rate
Sample Preparation and Injection
Column Testing
Storage
Maintenance
Agilent Ordering Information

Specifications

Typical operating pressure	900 psi (60 bar)
Maximum operating pressure	3700 psi (250 bar)
Maximum operating temperature	40 °C
Efficiency	> 75,000 plates

Frequently asked questions

What eluent pH range is recommended for ProSEC 3008 columns?

Buffered eluent systems within the pH range 5.0-7.5 are recommended.

Can organic solvents be used with ProSEC 3008 columns?

Yes, organic solvents and organic/aqueous mixtures may be used.

What is the recommended flow rate for a ProSEC 3008 column with 4.6 mm ID?

The recommended flow rate is 0.3 mL/min.

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