This note describes the analysis of albumin proteins using Agilent ProSEC 300S columns. Size exclusion chromatography (SEC) is used to investigate the aggregation of albumin proteins in solution, including dimers and trimers. This technique is of interest in biomedical applications.
The document details an experiment analyzing the aggregation of three specific albumin proteins: bovine serum albumin (BSA), lactalbumin, and ovalbumin. The analysis was performed using size exclusion chromatography (SEC) with Agilent ProSEC 3008 columns to investigate protein conjugates such as dimers and trimers.
The column dimensions are 300 x 7.5 mm.
The sample was analyzed at a flow rate of 1.0 mL/min.