The wide resolving range of the ProSEC 300S column is suitable for the analysis of a wide variety of proteins on the basis of their size in solution and molecul
This document details the use of polyethylene glycol/oxide standards to probe the molecular weight resolving range of the Agilent ProSEC 3008 column. The analysis involves a series of chemically and structurally homologous polymers to ensure accurate size exclusion data for protein samples.
| Column | ProSEC 3008, 300 x 7.5 mm |
|---|---|
| Eluent | 0.3M KH2PO4-K2HPO4, pH 6.8, containing 0.3M |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 20 uL |
| Sample Concentration | 4mg/mL |
| Temperature | 25°C |
| Detection | UV at 280 nm |
| Resolving Range | up to around 70,000 Daltons |
The column resolves up to around 70,000 Daltons.
The column dimensions are 300 x 7.5 mm.
The flow rate is 1.0 mL/min.