Key facts
- Brand
- Agilent
- Model
- 3100 OFFGEL Fractionator
- Documents
- 10
- Languages
- EN
- Last added
- 2012-07-05
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Agilent 3100 OFFGEL Fractionator Kit Quick Start Guide
This guide provides step-by-step instructions for preparing solutions and samples for protein and peptide fractionation using the Agilent 3100 OFFGEL Fractionator Kit. It includes reagent preparation, sample handling, and troubleshooting. The kit contains IPG strips, frames, electrodes, and reagents for isoelectric focusing. Best practices for sample preparation and equipment setup are detailed to ensure optimal performance.
The guide also covers how to assemble IPG strips, frames, and electrodes, and how to run and retrieve fractions. Safety precautions, such as wearing gloves and eye protection, are emphasized. Sample preparation varies based on strip size (12 cm or 24 cm) and sample type (protein or peptide).
Agilent 3100 OFFGEL Fractionator specifications
| Weight | 14 kg |
|---|---|
| Dimensions | 157 x 355 x 427 mm (height x width x depth) |
| Line Voltage | 100 — 240 VAC, +/- 10% |
| Power consumption | 140 VA |
| Flash Memory Card capacity | 512 MB storage capacity |
| Platform temperature range | 10 C-35C |
| Voltage Range (Power supplies) | 0.5-10kV |
| Fractionation capacity | 2 x 8 samples (12 or 24 fractions) |
Source: Agilent 3100 OFFGEL Fractionator Specifications Datasheet
All Agilent 3100 OFFGEL Fractionator documents by type
Ratings, dimensions and ordering codes, for selection and design work.
How to use the product for one specific task, with worked examples.
The shortest path to a working setup, usually only a few pages.
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
What is the maximum operating altitude for this instrument?
The instrument can operate at altitudes up to 2000 m (6500 ft).
From: Agilent 3100 OFFGEL Fractionator Specifications Datasheet
What is the loading capacity per sample?
The loading capacity ranges from 50 ug to 5 mg of total protein or peptide amount per sample.
From: Agilent 3100 OFFGEL Fractionator Specifications Datasheet
What is the resolution of the fractionation?
The system provides reproducible fractionation with a resolution as low as 0.1 pH.
How many samples can be processed in parallel?
The instrument allows for the fractionation of up to 16 samples in parallel on two trays.
What is the benefit of combining immuno-depletion with OFFGEL electrophoresis?
Combining these approaches allows for a more in-depth characterization of plasma samples, identifying two- to threefold more proteins and three- to fourfold more peptides compared to controls without fractionation.
Which abundant proteins are depleted from human plasma?
The method depletes six high abundant proteins: albumin, IgG, IgA, haptoglobulin, transferrin, and antitrypsin.