This is an application note that describes how to use Agilent's Polaris C18-A column to separate anti-inflammatory and analgesic (acidic) drugs.
This document is an application note detailing the high-performance liquid chromatography (HPLC) separation of a specific group of acidic drugs, including anti-inflammatory and analgesic agents. The procedure utilizes an Agilent Polaris C18-A column with dimensions of 150 x 4.6mm.
The method employs a gradient elution starting at 45% and increasing to 80% of solvent B (acetonitrile with 0.1% formic acid) over 10 minutes, with solvent A being water with 0.1% formic acid. The flow rate is set to 1.0 ml/min at ambient temperature, with detection performed using UV at 220 nm. The specific analytes listed for separation include Acetylsalicylic acid, Carbamazepine, Salicylic acid, Tolmetin, Indomethacin, Naproxen, Diclofenac, and Louprofen.
| Column | Polaris C18-A 5y 150 x 4.6mm |
|---|---|
| Column Part Number | A2000150X046 |
| Eluent A | H2O + 0.1% HCOOH |
| Eluent B | MeCN + 0.1% HCOOH |
| Gradient | 45 - 80%B in 10 min |
| Flow Rate | 1.0 ml/min |
| Temperature | ambient |
| Detection | UV @ 220 nm |
The note lists Acetylsalicylic acid, Carbamazepine, Salicylic acid, Tolmetin, Indomethacin, Naproxen, Diclofenac, and Louprofen.
The gradient increases from 45% to 80% of solvent B over a period of 10 minutes.