Agilent Polaris C18-A Column Application Note: Separation of Fat Soluble Vitamins

Polaris C18-A 3µ 150 x 2.0mm column for separation of fat soluble vitamins by high performance liquid chromatography.

Brand: Agilent
Document type: Application Note
Language: English
Pages: 1
File format: PDF
File size: 38 KB
Published: 12 June, 2012
Updated: 30 September, 2023
Model: C18-A
MD5 checksum: EEFAFBFFE4822D3A8E91AEC82E241BB4
PDF: Agilent Polaris C18-A Column Application Note: Separation of Fat Soluble Vitamins PDF

About this manual

This document provides experimental conditions for the High-Performance Liquid Chromatography (HPLC) separation of fat-soluble vitamins. The method utilizes a Polaris C18-A column with specific dimensions and part number A2001150X020.

The recommended eluent consists of MeCN containing 0.4% acetic acid, delivered at a flow rate of 0.3 ml/min at a temperature of 30°C. Detection is performed using UV detection at 280 nm.

What's inside

Vitamins
Polaris C18-A Column
HPLC Separation
Eluent Composition
Detection Conditions
Flow Rate and Temperature
Vitamin A, D2, D3, E

Frequently asked questions

What is the stated length and diameter of the Polaris C18-A column?

The column has a length of 150 mm and a diameter of 2.0 mm.

What is the specified flow rate for the separation procedure?

The flow rate is set to 0.3 ml/min.

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