This article describes the analysis of nucleotides using an Agilent Polaris C18-A column. The HPLC separation of ten nucleotides was achieved in 24 minutes. The column has a particle size of 5 μm and is suitable for pharmaceutical applications.
This document presents an HPLC separation method for ten nucleotides achieved in 24 minutes using an Agilent Polaris C18-A column. The method details the specific mobile phase composition, gradient profile, flow rate, and temperature conditions required for the analysis.
| Column dimensions | 4.6 x 150 mm |
|---|---|
| Particle size | 5 ym |
| Column part number | A2000150X046 |
| Flow rate | 1.5 mL/min |
| Detector wavelength | 254 nm |
| Analysis time | 24 minutes |
An HPLC separation of ten nucleotides was achieved in 24 minutes.
The flow rate specified for this analysis is 1.5 mL/min.