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Agilent 1120/6140 System Analysis of Fumonisin FB1 and FB2 Application Note
The document describes a method for detecting fumonisins (FB1 and FB2) in food and feed samples. It compares two extraction techniques: a simple extraction method (EM) and an immunoaffinity column (IAC) method, analyzing results using the Agilent 1120 Compact LC System coupled to an Agilent 6140 Single Quadrupole LC/MS System.
The study evaluates linearity, recovery rates, and matrix effects. Results indicate that while the EM method offers higher recoveries (62% to 78%) and is more cost-effective, the IAC method produces less matrix effect with lower recovery rates (42% to 64%). The chromatographic method demonstrated high linearity with an R² > 0.9999.
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How to use the product for one specific task, with worked examples.
Mounting, wiring and first power-up. Read it before the hardware goes in.
Questions answered in these manuals
What are the recovery ranges for the simple extraction method (EM)?
The recoveries from the EM method range from 62% to 78%.
From: Agilent 1120/6140 System Analysis of Fumonisin FB1 and FB2 Application Note
What is the linearity (R²) of the chromatographic method?
The chromatographic method is linear with an R² greater than 0.9999.
From: Agilent 1120/6140 System Analysis of Fumonisin FB1 and FB2 Application Note
How long does the installation process take?
The expected time for this procedure is about 2 hours.
What is the expected time to complete the installation of the Agilent 1120 Compact LC System?
The expected time for this procedure is about 2h.